Antidiabetic evaluation of vanillic acid-glyburide loaded polymeric micelles in high fat diet and streptozotocin induced diabetic rats

Abstract

Abstract Purpose The present study describes oral administration of glyburide (GLY) and vanillic acid (VA) loaded polymeric micelles for the effective treatment of type 2 diabetes mellitus in high-fat diet (HFD) and a low dose of streptozotocin (STZ) rats. Methods The rats were divided into 12 groups (G1-G12) based on the respective treatments. All rats except G1 received high fat diet for first 15 days and then induced to STZ. Then the study was continued for another 28 days. GLY and VA loaded polymeric micelles (GV-APMs) were orally administered to the STZ induced rats. The rats of different groups received their respective treatments orally. Various parameters such as body weight, blood glucose level, biochemical parameters and histopathology of liver and pancreas. The rats of G2 received HFD-STZ alone, whereas rats of G6 received high dose of mixture of raw Gly-VA and G12 received high doses of GV-APMs, separately. Results The results revealed 1.43, 1.44, 1.55, 2.4, 1.66, 3.30, and 3.76-folds higher reduction in blood glucose, cholesterol (CHL), triglycerides, low-density lipoprotein (LDL), very LDL levels, CHL/high-density lipoprotein (HDL), and LDL/HDL ratio in rats receiving GV-APMs (G12) than rats receiving their physical mixture (G6). In addition to this, significant reduction in the serum inflammatory, hepatic and renal markers as well as antioxidant levels was observed in rats of G12 as compared to any other group, indicating higher efficacy of GV-APMs. Conclusion The study revealed that co-loading of GLY and VA in APMs showed excellent antidiabetic potential can be explored further for clinical evaluation.