Potential Antibacterial Co-culture Streptomyces flavalbus from Indonesian mangroves induces the production of pyocyanin by Pseudomonas aeruginosa

Abstract

Abstract The potential Actinobacteria from the sediment and root Soneratia alba with co-culture as an antibacterial agent. 28 strains of Actinobacteria were found, composed of 17 strains from the sediment and 11 strains from the endophyte root of Sonneratia alba. Out of 580 fermentation, 540 fermenter co-cultures and 34 single fermenter cultures were assessed. Twenty-one pairs of co-culture were resulted positively against Staphylococcus aureus, and 16 pairs were positive against Pseudomonas aeruginosa. The highest clearance zone against S. aureus was obtained from 10 (Actino)-Aspergillus fumigatus (dead body) extract (21.57 ± 0.90 mm) while against P. aeruginosa was resulted from co-culture strains 3/37 extract (19.18 ± 0.80 mm). Strain 41 was determined to be Streptomyces flavalbus (98.07%) with the accession number MZ540332 when co-cultured with Pseudomonas aeruginosa to produce pyocyanin. Other Actinobacteria (6, 10, and 37) strains were identified as Streptomyces albogriseolus (99.79%), Streptomyces labedae (99%), and Streptomyces rochei (93.52%). It is concluded that co-culture is a valuable strategy to develop the new Actinobacteria product as a powerful antibacterial agent.