Potentials of Cellulose and Silica-based Materials for Enzyme Immobilization

Abstract

Abstract This research aims to develop biomaterials for a place for enzyme immobilization in a designed porous matrix. Hydrolyzed bacterial cellulose was entrapped in the silica rice husk surface during gelling to modify the surface properties. Immobilized enzymes were investigated for their life duration and reusability. In this research, cellulase, trypsin, and lipase enzymes were used as probe enzymes to be immobilized in the silica-cellulose matrix, and their activity was tested using spectroscopic techniques. The systems exhibited good reusability up to 6 cycles with the also interesting outcome from the dynamics in the interface, which were varied by temperature, pH, and stirring speed. Silica-cellulose was also compared to silica alone in this experiment. When physical interaction with surfaces was assumed, enzyme activity decreased to 10, 20, and 40% due to confinement but underwent complex dynamics due to speedy adsorption-desorption equilibrium.