Potentials of Cellulose and Silica-based Materials for Enzyme Immobilization-Reference-Cited by-同舟云学术

Potentials of Cellulose and Silica-based Materials for Enzyme Immobilization

Published:2022-12-09 Issue: Volume: Page:
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Author:

Wonorahardjo Surjani¹,Suharti Suharti¹,Yuniawati Nurakhma¹,Fatimasari Emada¹,Setyaningtyas Anis¹,Haula Hamizah¹,Susanti Evi¹,Molo Antonius DRP¹,Maharani Chariztya A¹,Sari Dian Puspita¹

Affiliation:

1. State University of Malang

Abstract

Abstract This research aims to develop biomaterials for a place for enzyme immobilization in a designed porous matrix. Hydrolyzed bacterial cellulose was entrapped in the silica rice husk surface during gelling to modify the surface properties. Immobilized enzymes were investigated for their life duration and reusability. In this research, cellulase, trypsin, and lipase enzymes were used as probe enzymes to be immobilized in the silica-cellulose matrix, and their activity was tested using spectroscopic techniques. The systems exhibited good reusability up to 6 cycles with the also interesting outcome from the dynamics in the interface, which were varied by temperature, pH, and stirring speed. Silica-cellulose was also compared to silica alone in this experiment. When physical interaction with surfaces was assumed, enzyme activity decreased to 10, 20, and 40% due to confinement but underwent complex dynamics due to speedy adsorption-desorption equilibrium.

Publisher

Research Square Platform LLC

Reference62 articles.

1. Silica-cellulose material application as the immobilisation matrix of Pseudomonas fluorescens,";Royanudin M;IOP Conf. Ser. Earth Environ. Sci.,2020

2. The application of silica-cellulose material as heavy metal adsorbent on laboratory wastewater;Royanudin M;May,2021

3. Spectroscopy for Characterization and Application of Silica-Cellulose Biomaterials, a Brief Review Article about Analytical Methods;Wonorahardjo S;IOP Conf. Ser. Mater. Sci. Eng.,2019

4. S. Wonorahardjo, F. Fariati, and I. W. Dasna, “Modern Analytical Chemistry Methods for Chalcogen Materials Analysis and Characterization,” in Chalcogen Chemistry, Londoon: Intech Open, 2018.

5. S. Wonorahardjo, A. R. Wijaya, and S. Suharti, “Surface Behavior of Rhodamin and Tartrazine on Silica-Cellulose Sol-Gel Surfaces by Thin Layer Elution,” J. Pure Appl. Chem. Res., vol. 5, no. May, pp. 48–54, 2016.