Enhancing Enzymatic Activity Through Co-Immobilization of Protease and Lipase Enzymes on Magnetic Nano-Iron Oxides Coated with Gum Arabic

Abstract

Abstract In this research, an easy method was followed for the co-immobilization of proteolytic and lipolytic enzymes on magnetic nanoparticles (MNPs) using magnetite (Fe3O4) nanoparticles to enhance the enzymatic activity. These oxides were obtained from Sky Spring Nanomaterials (U.S.A.) with a size of 30 nm, it was coated with gum arabic at a ratio of 0.05 mg/l of distilled water and the surface activation process was carried out using the binder glutaraldehyde at a concentration of 5%, then the lipase and protease enzymes prepared from Solarbio (China) and isolated from the Aspergillus niger mold. These enymes were restricted to the nanoparticles coated with gum arabic and activated with the binder at pH (7) and temperature 37°C. The immobilization efficiency of lipase and protease enzymes on these particles was (130, 108.86)% respectively. The effect of free and immobilized enzymes on the hydrolysis of fats and proteins was studied. The percentage of hydrolysis of fats and proteins was (3494.6, 2450.53) U/mg, respectively, for the immobilized enzymes on nanoparticles, and (2688, 2250.1) U/mg, respectively) for the free ones.