Characterization and antibacterial potential of Escherichia phage CMSTMSU isolated from shrimp farm effluent water

Abstract

Abstract Escherichia Phage CMSTMSU isolated from the semi-intensive shrimp farm was efficient lytic activity and strong host range against Escherichia coli. A High Density Polyethylene (HDPE) template was used to inhibit biofilm activity against E. coli, and results obtained using a confocal laser scanning microscope showed that the phage efficiently prevented biofilm formation. In order to assess the structural morphology, Escherichia Phage CMSTMSU was investigated using a transmission electron microscope at 80 kV and a magnification of X 12,000. The result revealed that, head measuring about 220–230 nanometer with a hexagonal shape and a non-contractile tail and the phage belongs to Siphoviridae family. By using a spectrophotometric assay to measure the killing efficiency, it was found that the phage significantly (P < = 0.001) contributed to a reduction in E. coli cell densities over time. Escherichia phage CMSTMSU's stability was tested at various temperatures and pH levels, and the results showed that the phage was stable at temperatures of 40 and 50˚C and pH levels of 5 to 9, respectively. These results were considerably (P < = 0.001) different from those of other temperatures and pH levels. The one-step growth curve showed a positive correlation, which indicated that the burst size grew considerably (P < 0.05) as the time increased.