Ki-67 pulmonary Immunoreactivity in silver nanoparticles toxicity: size-rate dependent genotoxic impact

Abstract

Abstract BackgroundEngineered nanoparticles have been recently utilized in numerous domains particularly, silver nanoparticles (AgNPs). Nonetheless, the possible side effects resulting from AgNPs exposure are not fully clarified. The present study aimed to clarify the toxicity of AgNPs toxic effect on lung tissue. This study was extended to investigate the impact of Glycosmis pentaphylla (G. pentaphylla) and Casimiroa edulis (C. edulis) leaves extracts in addition to mucilage and protein; the purified compounds from C. edulis against AgNPs induced pulmonary toxicity. MethodsMale Swiss albino mice were administered AgNPs orally in two different particle sizes (20 nm and 100 nm) for one month and was further treated via the above mentioned natural product extracts in a dose of 500 mg/ kg for three weeks. Biochemical, molecular, immunohistochemistry, and histopathological investigations were further assessed. ResultsOur results declared an obvious alternation in oxidative stress biomarkers as well as mRNA gene expression of both survivin and matrix metalloproteinase (MMP-9) in addition to exploration of positive nuclei for ki-67. Data declared a significant upregulation of both glutathione s transferase and superoxide dismutase antioxidants up on treatment by C. edulis extract. Furthermore, a remarkable downregulation of MMP-9as well as survivin mRNA gene expression in all treated groups. Immunohistopathological examination investigated a significant improvement in the reactivity of ki-67 biomarker upon treatment. Histopathlgical examination confirmed the obtained results.ConclusionIn conclusion; these functional foods extracts could be considered as a promising candidate as therapeutic regimen against pulmonary toxicity induced via Ag-NPs due to their enrichment with different active constituents.