Affiliation:
1. Center for Biomedical Research of La Rioja (CIBIR)
2. KU Leuven
3. Centro de Salud Siete Infantes de Lara
4. Hospital Universitario San Pedro
Abstract
Abstract
Introduction: Viruses are the most abundant components of the human gut microbiome with a significant impact on health and disease. The impact of human immunodeficiency virus (HIV) infection on the gut virome has been scarcely analysed and several studies suggested that integrase strand transfers inhibitors (INSTIs) are associated with a healthier gut. Thus, the objective of this work was to evaluate the effects of HIV infection and INSTIs on the gut virome composition. Methods: 26 non-HIV-infected volunteers, 15 naive HIV-infected patients and 15 INSTIs-treated HIV-infected patients were recruited and their gut virome composition was analysed using shotgun sequencing. Results: Bacteriophages are the most abundant and diverse viruses in gut. HIV infection was accompanied by a decrease in phage richness which was reverted after INSTIs-based treatment. β-diversity of phages revealed that samples from HIV-infected patients clustered separately from those belonging to the control group. Differential abundant analysis showed an increase in phages belonging to Caudoviricetes class in the naive group and a decrease of Malgrandaviricetes class phages in the INSTIs-treated group compared to the control group. Besides, it was observed that INSTIs-based treatment was not able to reverse the increase of lysogenic phages associated with HIV infection or to modify the decrease observed on the relative abundance of Proteobacteria-infecting phages. Conclusions: Our study describes for the first time the impact of HIV and INSTIs on the gut virome and demonstrates that INSTIs-based treatments are able to partially restore gut dysbiosis at the viral level, which opens several opportunities for new studies focused on microbiota-based therapies.
Publisher
Research Square Platform LLC
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