Energy metabolism, oxidative stress and immunological status in adult hip replacement patients and healthy individuals

Abstract

The work aimed to compare (1) the activity of energy metabolism enzymes and oxidative stress indicators in homogenates of the tensor fascia lata muscle with peripheral blood mononuclear cell (PBMC) isolates in patients with hip replacement (HRP) and (2) PBMCs energy and stress metabolism, and indicators of inflammation and immunity of HRP and healthy individuals. The study involved 16 HRP patients. Skeletal muscle and blood samples were taken during HR. Moreover, blood samples of 12 control subjects were used to isolate PBMCs. The activity of aerobic (malate dehydrogenase; MDH) and anaerobic (lactate dehydrogenase; LDH) metabolism enzymes and the level of carbonyl groups were measured in skeletal muscle homogenates and PBMC isolates. The indicators of inflammation and immunity were also assessed. The purity level of PBMCs isolates were determined using platelets to PBMC ratio. There was no relationship between MDH and LDH activities and carbonyl groups measured in skeletal muscle homogenate and PBMC isolate. Significantly higher inflammatory indexes (NLR and the number of neutrophils) and the level of protein carbonyl groups were also noted in the HRP group compared to the control group.