Calculation a new formula to determine the chlorophyll-a content without extraction by modeling meta analysis data in Arthrospira platensis Gomont

Abstract

Spectrophotometric methods are frequently used to monitor growth and development in algae cultures of toxicity studies. Chlorophyll-ameasurement is one of the most important parameters determining the growth in cyanobacteria cultures. In determining this parameter, primarily extraction with solvents and daily measurements should be carriedout during the study. The use of this method causes both labor and material consumption. Since the chlorophyll-acontent is related to the doubling time and initial culture concentration, it is possible to measure chlorophyll-aby determining these values by modeling. Mathematical model analysis has gained importance in phycology studies with the development of hardware and software depending on technology. Microbial processes modeling allows us to have information about the growth behavior of microalgae and to obtain effective results in the output of algal culture. For this purpose, the available data were adapted to Gompertz, Logistic, Weibull, Exponential and Richards models in multiple pesticide applications. By means of the regression formula obtained from the doubling time values of OD560 and chlorophyll-a, the chlorophyll-a can be easily determined by OD560 in forward studies.