Inactivation efficiency evaluation of four disinfectants for feline parvovirus derived from sick giant panda

Abstract

Abstract Background Feline panleukopenia (FP) is a highly contagious acute infectious disease caused by feline parvovirus (FPV). Parvovirus has a wide range of hosts, can infect many carnivores, and can remain infectious in the natural environment for a long time. Giant pandas are also susceptible to parvovirus. The viral enteritis of giant pandas caused by the virus infection poses a great threat to the survival of giant pandas. Therefore, screening out effective disinfectants is of great help for giant panda feeding management. Results The efficacy of four disinfectants against giant panda-derived FPV by determining viral titers, viral copies and viral hemagglutination after interacting virus with different disinfectants in the same amounts was evaluated. The virus titers decreased to 0 after treatment with peracetic acid (PAA), DuPont Virkon S (Virkon S) and glutaral and deciquam solution (JM). The inhibition by PAA was finally found to be most pronounced by determining viral copies. The virus hemagglutination titers decreased by 2 titers after 2 h of PAA treatment, and the virus hemagglutination titers decreased by 1 titer after Virkon S treatment. The immunofluorescence analysis showed that the PAA could effectively kill the virus within 1 h, while the JM and Virkon S took 2 h to achieve the killing effect. PAA, Virkon S, and glutaral and JM inhibited the production of viral structural protein within 1 h. Conclusions In this study, through multi-faceted comparison, the most effective disinfectant could be screened out, which could be used as a prevention and control disinfectant on panda origin feline panleukopenia virus, and provide reference opinions for giant panda disease control and prevention.