Affiliation:
1. University of Milan
2. Clemson University
3. Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
4. Texas A&M University
5. University of Arkansas System Division of Agriculture
Abstract
Abstract
Genomic regions associated with ripening time (RPT) and soluble solids concentration (SSC) were mapped using a pedigreed population including multiple F1 and F2 families from the Clemson University peach breeding program (CUPBP). RPT and SSC QTLs were consistently identified in two seasons (2011 and 2012) and the average datasets (average of two seasons). A target region spanning 10,981,971 − 11,298,736 bp on chromosome 4 of peach reference genome used for haplotype analysis revealed four haplotypes with significant differences in trait values among different diplotype combinations. Favorable alleles at the target region for both RPT and SSC were determined and a DNA test for predicting RPT and SSC was developed. Two Kompetitive Allele Specific PCR (KASP) assays were validated on 84 peach cultivars and 163 seedlings from the CUPBP, with only one assay (Ppe.RPT/SSC-1) needed to predict between early and late-season ripening cultivars and low and high SSC. These results advance our understanding of the genetic basis of RPT and SSC and facilitate selection of new peach cultivars with the desired RPT and SSC.
Publisher
Research Square Platform LLC
Reference47 articles.
1. Bassi, D., Mignani, I., Spinardi, A. & Tura, D. Chapter 23 - PEACH (Prunus persica (L.) Batsch). in Nutritional Composition of Fruit Cultivars (eds. Simmonds, M.S.J. & Preedy, V.R.) 535–571 (Academic Press, San Diego, 2016).
2. FAOSTAT. Food and Agriculture Organization of the United. FAOSTAT database: Data. Available via http://www.fao.org/faostat/en/#data/QV (2023).
3. Anthony, B.M. & Minas, I.S. Optimizing Peach Tree Canopy Architecture for Efficient Light Use, Increased Productivity and Improved Fruit Quality. Agronomy 11, 1961 (2021).
4. Differential effect of cultivar and harvest date on nectarine colour, quality and consumer acceptance;Iglesias I;Scientia Horticulturae,2009
5. Metabolic Profiling during Peach Fruit Development and Ripening Reveals the Metabolic Networks That Underpin Each Developmental Stage;Lombardo VA;Plant Physiology,2011