Affiliation:
1. USDA ARS
2. Boyce Thompson Institute
3. Bulgarian Academy of Sciences
4. Cotton Incorporated
5. Texas A&M University
6. Dovetail Genomics, LLC
Abstract
Abstract
Objective: The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), is a pest damaging many cultivated crops in North America. Although partial transcriptome data are available for this pest, a genome assembly was not available for this species. The aim of this was to assemble a high-quality chromosome-length genome of TPB to develop the genetic resources to provide foundation required for advancing research on this species.
Results:
The initial genome of TPB assembled with paired-end nucleotide sequences generated with Illumina technology was scaffolded with Illumina HiseqX reads generated from a proximity ligated (HiC) library to obtain a high-quality genome assembly. The final assembly contained 3968 scaffolds longer than 1 kbp to yield a genome of 599.97 Mbp. The N50 of the TPB genome assembly was 35.64 Mbp and 98.68% of the genome was assembled into 17 scaffolds larger than 1 Mbp. This megabase scaffold number is the same as the number of chromosomes observed in karyotyping of this insect. The TPB genome is known to have high repetitive DNA content, and the reduced assembled genome size compared to flowcytometric estimates of approximately 860 Mbp may be due to the collapsed assembly of highly similar regions.
Publisher
Research Square Platform LLC
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