Kinetics of protein extraction from excess sludge by thermal alkaline treatment

Author:

Hui Wenlong1,Zhou Jiti1,Jin Ruofei1

Affiliation:

1. Dalian University of Technology

Abstract

Abstract Protein can be extracted effectively from excess sludge by thermal alkaline process. During the extraction process, insoluble protein in sludge was turned into soluble protein, and part of the soluble protein was hydrolyzed into peptide. Then part of the soluble protein and peptide were degraded into amino acids, generating by-products: water, carbon dioxide and ammonium. Therefore, to obtain the maximum concentration of soluble protein, it is necessary to establish a kinetic model describing the process of sludge protein extraction. The report indicated that the extraction mechanism of sludge protein was according to first-order continuous reaction kinetic. A kinetic model of the reaction was established to explain the relationship between the rate constant, temperature and hydrolysis time of the first-order reaction. The optimum conditions were as follows: 0.1 M NaOH, reaction temperature 70°C, hydrolysis time 4 h. Protein separated and obtained from sludge hydrolysate by centrifugation and filtration, which could be used as a feed additive or foaming agent.

Publisher

Research Square Platform LLC

Reference40 articles.

1. Academy AS (2018) Determination of crude protein in feeds—Kjeldahl method. State Administration for Market Regulation, p 8

2. Measurement of Protein in 20 Seconds Using a Microwave Bca Assay;Akins RE;Biotechniques,1992

3. Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge;Boleij M;Environ Sci Technol,2018

4. Solubilization and characterization of extracellular proteins from anammox granular sludge;Boleij M,2019

5. Effects of alkaline solvents and heating temperatures on the solubilization and degradation of gel-forming Extracellular Polymeric Substances (EPS) extracted from aerobic granular sludge;Bou-Sarkis A;Biochem Eng J,2022

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