Characterization of Antimicrobial Peptide Produced by Bacillus Subtilis Subsp. Subtilis

Abstract

Abstract Purpose: Antimicrobial peptides are amongst the most promising class of peptides to contract the rise of global antimicrobial resistant. This article investigates a new antimicrobial peptide from bacteria.Methods: Bacterial identification was based on phenotypical and biochemical properties as well as 16S rRNA gene sequence homology. Bacterial growth and production of the inhibitory substance was standardized and optimized. The newly antimicrobial peptide was purified to homogeneity, subsequently analyzed by PAGE and MALDI-TOF-MS.Results: The bacterium identified as Bacillus subtilis subsp. subtilis and designated as strain MZ-32. Landy medium was best for the production when compared with different media after fixing the least influential variables in standardized fermentation conditions. Carbohydrate and nitrogen supplements investigated to improve production in Landy medium. The antimicrobial peptide of 2.158-Da was active against a broad range of skin-born pathogenic bacteria that were resistant to standard antibiotics, and possessed the physico-chemical properties of an ideal antimicrobial agent in terms of water solubility, thermal resistance, and stability towards acid/alkali (pH 4.0 to 9.0) treatments.Conclusion: The new strain and its associated peptide are potentially new candidates for medical and biotechnological applications.