LncRNA PCSK1N-202 regulates CD34 + hematopoietic cells proliferation in MDS patients via PIM2/HIF-1alpha

Abstract

Abstract Background Myelodysplastic syndromes (MDS) are characterized by malignant clonal hematopoietic stem cells with high-risk of progression to acute myeloid leukemia (AML).Results In this study,we explore the role of PIM2/HIF-1alpha in the proliferation of CD34 + cells in MDS patients. We investigate the profile of lncRNA derived from the bone marrow CD34 + cells in MDS patients and AML secondary to MDS (sAML) patients. We found 1173 lncRNAs (648 upregulated/525 downregulated) and 326 mRNAs (125 upregulated/201 downregulated) significantly deregulated in sAML compared to those in MDS patients (|logFC| > 1, p value < 0.05). Of these, lncRNA PCSK1N-202 predicted as targeted-regulator to PIM2.RT-PCR showed that the level of PCSK1N-202 was upregulated in AML and MDS patients and positive related with PIM2.For further demonstrated the regulation of PCSK1N-202 to PIM2,RNA fluorescence in situ hybridization indicated that PCSK1N-202 was distributed in the nucleus and cytoplasm which as well as PIM2.For functional study, SKM-1 cell was transfected with LV- PCSK1N-202 ,which induced the decline of PIM2 and HIF-1α followed with decreased of cell proliferation and increased of apoptosis.Conclusion In conclusion, the upregulation of PCSK1N-202 transcript may through PIM2 / HIF-1α regulate the proliferation of CD34 + cells in MDS.