Coagulation activity of Chitin Binding Protein to the Turbid Pond Water from Garden Candytuft Seeds-Reference-Cited by-同舟云学术

Coagulation activity of Chitin Binding Protein to the Turbid Pond Water from Garden Candytuft Seeds

Published:2024-09-10 Issue: Volume: Page:
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Author:

Shehzadi Ulfat¹,Rehman Afeefa¹,Khaliq Binish¹,Shah Anis Ali²,Iftikhar Muhammad²,Akrem Ahmed³,Shahzadi Bismillah¹,Shaffique Shifa⁴,Gatasheh Mansour K.⁵

Affiliation:

1. University of Okara

2. University of Education Lahore

3. Bahauddin Zakariya University

4. Kyungpook National University

5. King Saud University

Abstract

In advanced nations, reservoir water is frequently utilized for consumption or domestic use, and its turbidity is higher during wet seasons. It requires many artificial coagulants, which increases the cost of therapy. Finding natural alternatives, particularly those derived from plants, has become more relevant because of the negative health and environmental implications of traditional synthetic substances used in water clarification. In this study, a natural coagulant protein called chitin binding protein (CBP) purified from the seeds of Iberis umbellata L. was utilized to treat water and remove turbidity. To increase the effectiveness of turbidity removal, this study focuses on the extraction, isolation, and purification of natural coagulants from the seeds of I. umbellata L. At pH 7, the maximum turbidity reduction effectiveness was attained. The molecular weight of CBP was approximately 14 kDa, as revealed by SDS-PAGE and purified by spin column chromatography. The CBP of I. umbellata showed coagulation activity against synthetic clay solution and pond water. The coagulation results showed that CBP was 87% and Moringra extract showed 89% activity against the synthetic clay solution after 120 min. CBP from I. umbellata showed 83% and Mo extract 82% coagulation activity against the pond water. CBP from I. umbellata may be a viable natural coagulant for water treatment based on its coagulation performance against severely murky pond water.

Publisher

Springer Science and Business Media LLC

Reference35 articles.

1. 1. Armeloni, J. P. N., de Oliveira, D. S. & Donadel, C. B. Natural Agents as Auxiliaries in Water Clarification: Literature Review and Experimental Evaluation. Acta Sci. Technol. 42, e44800 (2020).

2. 2. Bichi, M. H. A. Review of the Applications of Moringa oleifera Seeds Extract in Water Treatment. Civ. Environ. Res. 3, 10 (2013).

3. 3. Delelegn, A., Sahile, S. & Husen, A. Water Purification and Antibacterial Efficacy of Moringa oleifera Lam. Agric. Food Secur. 7, 10 (2018).

4. 4. Pritchard, M., Mkandawire, T., Edmondson, A., O’Neill, J. G. & Kululanga, G. Potential of Using Plant Extracts for Purification of Shallow Well Water in Malawi. Phys. Chem. Earth Parts ABC 34, 799–805 (2009).

5. 5. Pritchard, M., Craven, T., Mkandawire, T., Edmondson, A. S. & O’Neill, J. G. A Comparison between Moringa oleifera and Chemical Coagulants in the Purification of Drinking Water—An Alternative Sustainable Solution for Developing Countries. Phys. Chem. Earth Parts ABC 35, 798–805 (2010).