Diversity of fungal community andquality evaluation of Spatholobus suberectus Dunn during the process of mildew

Abstract

Abstract Spatholobus suberectus Dunn as a traditional Chinese herbal medicine, which is susceptible to being infected by molds during storage. In order to explore the diversity characteristics of fungal community and the quality evaluation of Spatholobus suberectus Dunn during the process of mildew. The study used high-throughput sequencing technology to detect the diversity characteristics of fungal community, high-performance liquid chromatography (HPLC) and ultraviolet spectrophotometry (UV-spectrophotometry) methods to detect the content of flavonoids, and enzyme-linked immunosorbent assay (ELISA) method to detect the content of Aflatoxins B1 (AFB1). The result showed that the fungi of Spatholobus suberectus Dunn samples during the process of mildew belonged to 14 phyla, 43 classes, 92 orders, 189 families, 336 genera, 432 fungal taxa. The species diversity of fungal community was the highest at the early stage of mildew, and the species richness of fungal community was the highest at the late stage of mildew. At the genus level, the dominant fungus at the early stage of mildew was not obvious, while the dominant fungus of samples at middle and late stages of mildew was Aspergillus. The content of AFB1 showed an upward trend and the content of flavonoids showed a downward trend during the process of mildew. In brief, the diversity of fungal community decreased gradually, the number of dominant flavus increased gradually, and the quality of Spatholobus suberectus decreased gradually during the process of mildew.