Abstract
This study compared and analyzed the diversity differences of soil microbial communities in the rhizosphere of Panax notoginseng under different habitats (under forest and field) and their correlation with environmental factors. Illumina MiSeq high-throughput sequencing revealed that the diversity index of rhizosphere bacterial communities in P. notoginseng under forest (Ace, Chao1, Simpson, Shannon) was significantly higher than in field. In comparison to the microbial networks observed in the field (Nodes=186, Edges=3407), the microbial networks found in the underforest (Nodes=266, Edges=4549) demonstrate a more intricate and sophisticated correlation among microorganisms. The results revealed that organic carbon emerged as the primary main influencing the assembly of rhizosphere bacterial communities in P. notoginseng under forest, accounting for 84.23% of the observed community variation. However, in fungi, the pH (75.10 %) value assumes significance. Moreover, the total saponin content of P. notoginseng in the forest was significantly higher compared to that in the field, particularly with regards to saponin R1, which exhibited a significant association with the microbial community present under forest conditions.