Exosomes from Adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation

Abstract

Abstract Exosomes from Adipose-derived stem cells (ADSCs) have been demonstrated to benefit angiogenesis, wound healing and fat grafting. Small noncoding RNAs such as microRNA (miRNA) and circular RNA play critical role mediating the function of ADSC-derived exosomes. However, the underlying mechanisms have not been fully elucidated. In this study, we investigated the function and mechanism of ADSC-derived exosome promoting fat graft angiogenesis and adipogenic differentiation. Exosomes from ADSCs were isolated and identified while treatment with ADSC-derived exosomes enhanced fat graft angiogenesis and adipogenic differentiation in mouse fat graft implantation model. We found that ADSC-derived exosome overexpressed miR-671-3p and promoted human umbilical vein endothelial cell (HUVEC) proliferation, migration and invasion. Bioinformatics analysis and luciferase reporter assay validated that TMEM127 was a direct target of miR-671-3p. Rescue experiments demonstrated that overexpression of TMEM127 partially antagonized the function of ADSC-derived exosome in vitro, suppressing HUVEC cell proliferation, migration and invasion. Moreover, overexpression of TMEM127 abrogated the function of ADSC-derived exosome on fat graft angiogenesis and adipogenic differentiation. Taken together, our findings demonstrate that exosomes from ADSC overexpressing miR-671-3p promote fat graft angiogenesis and adipogenic differentiation.