Hexavalent chromium detoxification by haloalkaliphilic Nesterenkonia sp strain NRC-Y immobilized in different matrices

Abstract

Abstract In order to develop a bioprocess for Cr(VI) detoxification in industrial effluent, a previously isolated potent Cr(VI) reducing haloalkaliphilic Nesterenkonia sp strain NRC-Y was immobilized in nine different matrices including natural polymer, modified natural, synthetic and mixtures of natural and synthetic polymers and investigated for Cr(VI) detoxification. Among the tested carriers and immobilization approaches, Nesterenkonia sp NRC-Y cells encapsulated in amidated pectin beads showed highest Cr(VI) reduction efficiency (58.4% of initial Cr(VI) concentration 150 mg/L after 20 h), followed by alginate, alginate-PVA, alginate-PVA-chitosan, PVA-PVP, and PVA respectively. Therefore, amidated pectin was selected for further investigation and immobilization of both whole cells and chromate reductase. Operational stability study revealed that immobilized whole cell was more efficient and stable than immobilized chromate reductase and the free cells retaining about 60%, 27.0% and 11.5% of its initial activity after four successive batches, respectively. The temperature and pH optima for the immobilized cells were 35⸰C and 7.0, respectively. The pH and thermal stability of Nesterenkonia sp NRC-Y cells were significantly enhanced upon immobilization in amidated pectin beads. The developed immobilized biocatalyst was applied for Cr(VI) reduction in industrial effluent samples, and was able to completely reduce Cr(VI) within 4 and 8 h for effluents for initial Cr(VI) concentrations of 10 and 30 mg/L, respectively. To the best of our knowledge, this is the first report about of Cr(VI) detoxification by immobilized Nesterenkonia sp NRC-Y in amidated pectin beads. The developed immobilized biocatalyst is promising and has the potential for large-scale Cr(VI) detoxification application.