Effect of bleeding to Actinidia arguta (Sieb. & Zucc) Planch. ex Miq. on plant growth, physiological characteristics and fruit quality

Abstract

Abstract Bleeding, as particularly serious phenomenon in A. arguta, has an important effect on plant growth and development. In the study, to study the effects of bleeding on the growth and development of leaves and fruits after the occurrence of bleeding, we chose as Actinidia arguta (Sieb. & Zucc) Planch. ex Miq. Results revealed that bleeding damaged the leaf structure. The physiological indexes of plants after bleeding were detected and analyzed. The relative electrical conductivity and malondialdehyde (MDA) of leaves increased in treatment. Nitro blue tetrazolium chloride (NBT) and 3,3-diaminobenzidine (DAB) staining showed that reactive oxygen species (ROS) accumulation was obvious in leaves after bleeding. The chlorophyll content, Tr (transpiration rate), Pn (net photosynthetic rate), Ci (intercellular CO2 concentration) and Gs (stomatal conductance) of plants were also decreased. The pulp and the seed water content decreased after the damage. The fruit vitamin C, soluble sugar content and soluble solids content (SSC) decreased after the damage, but the titratable acid content had no significant difference. Therefore, the bleeding will affect the physiological indexes of A. arguta, and our study lay a theoretical basis for understanding the physiological changes of A. arguta after bleeding.