Long-time high-sucrose intake induced metabolic dysfunction via disrupting the balance of intestinal microenvironment

Abstract

Abstract Sucrose, a common sweeter, is frequently added to drink and food, and excess intake of sucrose is really harmful to our health. This study aimed to reveal the potential mechanisms of high-sucrose induced metabolic dysfunction. Sixty mice were divided into two groups, respectively, the normal group (Nor group) and high-sucrose group (HS group). 16S rDNA and untargeted metabolomics technologies were used to analyzed the dynamic changes of gut microbiota and metabolites in colon contents of young mice, middle age mice and old mice. Long-time high-sucrose intake induced fat deposition and metabolic disorder, along with a completely different gut microbiota map. The energy metabolism related gene expression of the gut microbiome was downregulated, but potential pathogen gene expression was upregulated by long-time high-sucrose intake. Furthermore, mice in the HS were utilized less amino acids and long chain fatty acids, with effecting tryptophan metabolism by producing less indole, more 5-HT and kynurenine. Also less secondary bile acids, and more TMA/TMAO were produced by gut microbes in HS group. These results demonstrated that long-time high-sucrose intake leads to fat deposition and metabolic disorder via disrupting the balance of intestinal microenvironment.