Caspase-3 driven uterine apoptotic changes during arsenic intoxication attenuated by the n-butanol fraction of Moringa seed: interplay informatics of caspase-3 with glucomoringin vs. methyl glucosinolate

Abstract

Abstract Background- Replacing invasive chelating therapy by non-invasive oral therapy against arsenic poisoning is indispensable because of the side effects of chelating agents. Aim- The present study was framed to achieve whether n-butanol fraction of Moringa oleifera seed (NB) could repair utero-damages following arsenisation. Methods- The arsenic-treated (10 mg/kg BW) rats received a dose of 50 mg/kg NB. The possible active components present in NB were investigated via HPLC-MS. Analysis of enzymatic antioxidants was accomplished by native gel electrophoresis. Pro and anti-inflammatory indicators were assessed by RT-PCR, and Western blot. ESR-α was detected via immunostaining. Results- Arsenicated rats showed significant augmentation in lipid peroxidation with decreased antioxidant enzymes activities which were considerably reversed by NB administration. Weak ESR-α expression along with distorted uterine histomorphology were retrieved by NB. Altered protein expression along with mRNA expression of inflammatory and apoptotic markers were also significantly recovered by NB. Molecular docking predicted that glucomoringin and methyl glucosinolate of Moringa interacts with the catalytic site of caspase-3 in the way of limiting its activity. Conclusion- NB was successful in restoring the arsenic-mediated uterine hypo-function. The glucomoringin and methyl glucosinolate presents in n-butanol fraction may play a critical role in limiting apoptotic event in arsenicated uterus.