Neiella litorisoli sp. nov., an alginate lyase – producing bacterium from South China Sea

Abstract

AbstractA Gram-stain-negative, rod-shaped bacterium, designated HB171785T, was isolated from soil sample collected from Qishui Bay, Hainan, China. The strain grew optimally at pH 7–8, 40–45°C and with NaCl 3–4%. The predominant isoprenoid quinone was found to be Q-8 and the major fatty acids were C16:0, C16:1ω7c/ C16:1ω6c, C18:1ω7c/ C18:1ω6cand C12:03OH. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The size of the draft genome was 4.32 Mbp with G + C content 49.7%. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain HB171785Tbelonged to the orderAlteromonadales, and the closest phylogenetically related species wereNeiella marinaj221T, “Neiella holothuriorum” 126 andEchinimonas agarilyticaLMG2520Twith the similarities of 98.2, 96.0 and 95.0%, respectively. The phylogenetic tree based on 16S rRNA genes and phylogenomic tree based on core genomes showed that strain HB171785Tclustered together withN.marinaj221T, with the highest values of average nucleotide identity (82.9%) and digital DNA-DNA hybridization (25.4%). The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB171785Trepresents a novel species of the genusNeiella, for which the nameNeiella litorisolisp. nov. is proposed. The type strain is HB171785T(= MCCC 1K04625T = KCTC 82319T).