Affiliation:
1. Institute of Chemistry, Technology and Metallurgy, Belgrade
2. Faculty of Chemistry, Belgrade
3. Faculty of Chemistry, Belgrade + Bremgroup, Belgrade
Abstract
The group of polycyclic aromatic hydrocarbons (PAHs) are very hazardous
environmental pollutants because of their mutagenic, carcinogenic and toxic
effects on living systems. The aim of this study was to examine and compare
the ability and efficiency of selected bacterial isolates obtained from
oil-contaminated areas to biodegrade PAHs. The potential of the bacteria to
biodegrade various aromatic hydrocarbons was assessed using the
2,6-dichlorophenol-indophenol assay. Further biodegradation of PAHs was
monitored by gravimetric and gas-chromatographic analysis. Among the eight
bacterial isolates, identified on the basis of 16S rDNA sequences, two
isolates, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, had the ability
to grow on and utilize almost all examined hydrocarbons. Those isolates were
further examined for biodegradation of phenanthrene and pyrene, as single
substrates, and as a mixture, in vitro for ten days. After three days, both
isolates degraded a significant amount phenanthrene, which has a simpler
chemical structure than pyrene. Planomicrobium sp.RNP01 commenced
biodegradation of pyrene in the PAH mixture only after it had almost
completly degraded phenanthrene. The isolated and characterized bacteria,
Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, have shown high
bioremediation potential and are likely candidates to be used for degradation
of highly toxic PAHs in contaminated areas.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
Subject
General Chemical Engineering
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献