Anticancer and apoptotic effects of a polysaccharide extract isolated from Lactarius chrysorrheus Fr. in HepG2 and PANC-1 cell lines-Reference-Cited by-同舟云学术

Anticancer and apoptotic effects of a polysaccharide extract isolated from Lactarius chrysorrheus Fr. in HepG2 and PANC-1 cell lines

Published:2022 Issue:4 Volume:74 Page:315-324
ISSN:0354-4664
Container-title:Archives of Biological Sciences
language:en
Short-container-title:ARCH BIOL SCI BELGRA

Author:

Mutlu Dogukan¹,Cakir Cansel²,Ozturk Mehmet²,Arslan Sevki¹

Affiliation:

1. Department of Biology, Faculty of Science, Pamukkale University, Denizli, Turkey

2. Deparment of Chemistry, Faculty of Science, Muğla Sıtkı Koçman University, Mugla, Turkey

Abstract

Mushrooms are widely used in many cultures for nutritional and health benefits. The Lactarius species is found in the Aegean region of Turkey. Lactarius chrysorrheus Fr. is a wild mushroom that contains a milky juice. In this study, we investigated the in vitro cytotoxic potential and apoptotic effect of the polysaccharide extract prepared from L. chrysorrheus by water extraction and alcohol precipitation using the tetrazolium MTT dye assay, annexin V staining, wound healing and colony formation, and qRT-PCR. The molecular weights of three peaks observed in HPLC chromatograms were calculated as 1869.9, 3043.92 and 16821.47 Da. The extract exhibited cytotoxic activity at 72 h, with an IC50 value of 296.42 ?g/mL in HepG2 and 444.43 ?g/mL in PANC-1 cells; the extract that was tested on the normal HEK293 cell line exhibited no cytotoxicity. Further, L. chrysorrheus upregulated the expression of CASPASE 3 and CASPASE 9 while downregulating B-cell lymphoma 2 (BCL-2) and B-cell lymphoma-extra large (Bcl-xL) genes, and inhibited cell migration and colony formation in HepG2 and PANC-1 cells. This study provides new insight into the use of the polysaccharide from L. chrysorrheus in the development of novel anticancer agents.

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

Reference58 articles.

1. Bains A, Chawla P, Kaur S, Najda A, Fogarasi M, Fogarasi S. Bioactives from Mushroom: Health Attributes and Food Industry Applications. Materials (Basel). 2021;14(24):7640. https://doi.org/10.3390/ma14247640

2. You SW, Hoskin RT, Komarnytsky S, Moncada M. Mushrooms as Functional and Nutritious Food Ingredients for Multiple Applications. ACS Food Sci Tech. 2022;2(8):1184- 95. https://doi.org/10.1021/acsfoodscitech.2c00107

3. Rangel-Vargas, E, Rodriguez JA, Dominguez R, Lorenzo JM, Sosa ME, Andres SC, Rosmini M, Perez-Alvarez JA, Teixeira A, Santos EM. Edible Mushrooms as a Natural Source of Food Ingredient/Additive Replacer. Foods. 2021;10(11):2687. https://doi.org/10.3390/foods10112687

4. Valverde ME, Hernandez-Perez T, Paredes-Lopez O. Edible Mushrooms: Improving Human Health and Promoting Quality Life. Int J Microbiol. 2015;(14):376387. https://doi.org/10.1155/2015/376387

5. Anusiya G, Gowthama PU, Yamini NV, Sivarajasekar N, Rambabu K, Bharath G, Banat F. A review of the therapeutic and biological effects of edible and wild mushrooms. Bioengineered. 2021;12(2):11239-68. https://doi.org/10.1080/21655979.2021.2001183

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Exploring 2-Pyrazoline derivatives as potent antidiabetic agents and cholinesterase inhibitors: Their synthesis and molecular docking studies;Journal of Molecular Structure;2024-11

2. Cytotoxic and Apoptosis-Inducing Activities of Iridium Complexes Bearing 2-Phenylimidazo[4,5-f][1,10]-Phenanthroline Derivatives in Human Cancer Cells;Pharmaceutical Chemistry Journal;2024-05

3. A review for cancer treatment with mushroom metabolites through targeting mitochondrial signaling pathway: In vitro and in vivo evaluations, clinical studies and future prospects for mycomedicine;Fitoterapia;2024-01

4. Biological activities of Liquidambar orientalis: Antibiofilm, cytotoxicity, apoptosis, and miRNA expressions;Botanica Serbica;2024

5. Polyacrylonitrile (PAN)/carbon nanotube (CNT) electrospun nanofibers: synthesis, characterization, their biocompatibility for L929 fibroblast cells and molecular docking studies;International Journal of Polymeric Materials and Polymeric Biomaterials;2023-12-23