Affiliation:
1. Institute for Medicinal Plant Research “Dr. Josif Pančić”, Belgrade
2. Insitute for Chemistry, Technology and Metalurgy, Belgrade
Abstract
Phenolic compounds are very important quality parameters of wine because of
their impact on colour, taste and health properties. The present study aimed
to evaluate the general phenolic composition and free radical scavenging
activity of aqueous and organic fractions obtained using liquid/liquid
extractions from red wine produced from Serbian autochtonous grape variety
Prokupac. Total phenolic contents in different fractions ranged from 48.22 to
289.12 mg GAE/g dry fraction. Phenolic acids (mainly hydroxycinnamic acids)
and quercetin-3-glucuronide were the main components of the EtOAc fraction at
pH 2.0; catechins, phenolic acids (mainly hydroxybenzoic acids) and quercetin
were found in the EtOAc fraction at pH 7.0, while anthocyanins were
identified in the aqueous residue after EtOAc extraction. The major
anthocyanin extracted into aqueous fraction was malvidin-3-glucoside, while
the most abundant non-anthocyanin phenolic compounds from organic fractions
were ethyl gallate and trans-caftaric acid. Radical scavenging activity
differed significantly among fractions and IC50 values were 138.58 ?g/ml for
water fraction, 17.83 and 3.47 ?g/ml for EtOAc fractions at pH 2.0 and pH
7.0, respectively. As the EtOAc fractions were found to be more potent
radical scavengers, it could be assumed that non-anthocyanin phenolic
compounds were responsible for such activity in Prokupac wine.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
Cited by
8 articles.
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