Immobilization of glucoamylase on macroporous spheres-Reference-Cited by-同舟云学术

Immobilization of glucoamylase on macroporous spheres

Published:2004 Issue:35 Volume: Page:207-214
ISSN:1450-7188
Container-title:Acta Periodica Technologica
language:en
Short-container-title:ACTA PERIOD TECHN

Author:

Milosavic Nenad¹,Prodanovic Radivoje²^ORCID,Jovanovic Slobodan³,Vujcic Zoran²

Affiliation:

1. Technology and Metallurgy, Belgrade

2. Faculty of Chemistry, Belgrade

3. Faculty of Technology and Metallurgy, Belgrade

Abstract

Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70?C, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours.

Publisher

National Library of Serbia

Subject

General Engineering

Reference15 articles.

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4. Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads

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