Stimulating effects of different basal media and cytokinine types on regeneration of endemic and endangered Muscari aucheri

Abstract

In this study a simple protocol has been developed for the endemic and endangered Muscari aucheri plant. 2-4 bulb scale explants of M. aucheri were cultured in different basal media (Nitsch mineral salts and vitamins, Orchimax Medium mineral salts and vitamins) supplemented with 2 mgL-1 2,4-D, 20 mgL-1 mannitol, 20 mgL-1 sucrose, 0.5 mgL-1 NAA and different concentrations of BAP, KIN, 2iP and TDZ. The medium was solidified with 2 mgL-1 Gelrite. The Orchimax and Nitsch & Nitsch medium promoted shoot and bulblet regeneration. BAP, KIN and 2-iP were suitable for induction of the bulblet for 2-4 bulb scales of Maucheri. The highest number of bulblets per explant for 2 and 4 scales was achieved on the Orchimax medium supplemented with 1 mgL-1 KIN and 2 mgL-1 KIN, respectively. Whereas, the maximum number of bulblets per explant for 4 scales was recorded on the Nitsch medium supplemented with 2 mgL-1 BAP. However, the Nitsch medium containing 10 mgL-1 2-iP gave the best number of bulblets per explant for 2 scales. Bulblets acclimatized very well under greenhouse and garden conditions. .