Discrimination and classification of tobacco wastes by identification and quantification of polyphenols with LC-MS/MS

Abstract

The chemical composition of polyphenols in tobacco waste was identified by HPLC-PAD-ESI/MS/MS and the contents of chlorogenic acids and rutin in 10 varieties of tobacco wastes were determined by HPLC-UV. The relationships between the contents of active polyphenols and the varieties of tobacco wastes were interpreted by hierarchical cluster analysis (HCA) and principal component analysis (PCA). The results showed that 15 polyphenols were identified in a methanolic extract of dried tobacco waste. The tobacco wastes were characterized by high levels of chlorogenic acids (3-CQA, 5-CQA, and 4-CQA) and rutin; their ranges in the 10 tobacco varieties were 0.116-0.196, 0.686-1.781, 0.094- 0.192, and 0.413-0.998 %, respectively. According to multivariate statistics models, two active compound variables can be considered important for the discrimination of the varieties of tobacco wastes: chlorogenic acids and rutin. Consequently, samples of 10 tobacco varieties were characterized into three groups by HCA based on the PCA pattern. In conclusion, tobacco waste could be used as a new pharmaceutical material for the production of natural chlorogenic acids and rutin in the ethnopharmacological industry.