Ксилотрофні агарикоміцети – антагоністи рідкісного гриба Hericium Coralloides (Scop.) Pers. (Hericiaceae) в культурі

Abstract

<p>We established the patterns of growth and type of mutual relations of <em>Hericium</em><em> с</em><em>oralloides</em><em> </em>2332 culture from PDA with the presence cultures of <em>Fomes</em><em> </em><em>fomentarius</em><em> </em>1528 and/or <em>Schizophyllum</em><em> </em><em>commune</em> 1763. It is established that cultures of <em>F. fomentarius</em>, і <em>S</em><em>. </em><em>commune</em> are antagonists to <em>H. сoralloides</em> on the PDA by direct confrontation and consecutive combined cultivation culture method. But, during direct confrontation mycelium of <em>H. сoralloides</em><em> </em>cultivation with culture <em>F. fomentarius</em> and/or <em>S</em><em>. </em><em>commune </em>the inhibition effect was 60-84% on the 10^th day of the experiment with stopping growth of <em>H. сoralloides</em>. In the case of consecutive combined cultivation culture method, the inhibition effect was 15-35%. Mycelium of <em>H. сoralloides</em><em> </em>was growing to contact with antagonists. The obtained results indicate the importance for the growth and survival of <em>H. coralloides</em> mycelia of primary occupation of the nutrient medium in binary and triple compositions. Between <em>H. coralloides</em> and <em>F. fomentarius</em> for direct confrontation the fact of bilateral antagonism is established. For the triple composition of the selected cultures, the phenomenon of additive antagonism is characterized. For cultures of the composition the <em>S. commune + F. fomentarius</em> on the 10^th day of the experiment, the fact of equilibrium confrontation was established. In direct confrontation for composition <em>H. coralloides + F. fomentarius</em> colonial characteristic was bilateral growth inhibition at a distance from the formation of inhibition zones. Colonies from binary composition <em>F. fomentarius + S. commune</em> and triple <em>H. coralloides + F. fomentarius + S. commune</em> for direct confrontation growth stopped after contact. For variant <em>H. coralloides + S. commune</em> growth has been a stop of the colonies <em>H. coralloides</em> followed the growth of mycelium <em>S. commune</em>. In a series of consecutive combined cultivation culture method for binary and triple composition of cultures after contact the colonies of <em>H. coralloides</em> growth stopped, rare mushroom the colony continued to grow over antagonist’s mycelium, but never overgrown it completely. According to the growth coefficients on PDA the monocultures <em>S. commune</em> and <em>F. fomentarius</em> belong to those growing at an average speed under the given experimental conditions, <em>H. coralloides</em> culture is slowly growing. These results are important for the remediation <em>H. coralloides</em> in vivo to avoid the loss of seed inoculum of <em>H. coralloides</em>. Fact antagonism between <em>H. coralloides</em> and <em>F. fomentarius</em> must be viewed for logs <em>Fagus silvaticus</em> involved to remediation <em>H. coralloides</em>.</p>