AN EFFECTIVE WAY TO CARRY OUT MASS IN VITRO PROPAGATION OF POTENTILLA ALBA L.

Abstract

<p>Despite the plant’s extensive area of distribution, <em>Potentilla alba </em>L.<em> </em>natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using <em>P. alba</em>, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of <em>P. alba</em> using <em>in vitro</em> culture in order to obtain a high-quality planting material. At the explant stage, the technique of the <em>P. alba</em> introduction into <em>in vitro</em> culture is developed. This paper reveals the morphogenetic features of the development of <em>P. alba</em> explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into <em>ex vitro</em> conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.</p>