Affiliation:
1. Institute of Human Ecology – branch of the Federal Research Center for Coal and Coal Chemistry of the Siberian Branch of the Russian Academy of Sciences
2. Kuzbass Clinical Oncology Dispensary
3. Institute of Fundamental Sciences, Kemerovo
State University
4. Kemerovo Regional Blood Center
5. Kuzbass Clinical Diagnostic Center named after A.I. I.A. Kolpinsky
Abstract
The study was aimed to determine the level of class G antibodies specific to Bp, Es, Pg (IgG-Bp, IgG-Es, IgG-Pg) in patients with colorectal and breast cancers. Material and methods. The content of these antibodies in the blood serum of healthy women (n=401), patients with colorectal cancer (n=219) and patients with breast cancer (n=1469) was studied using a non-competitive enzyme immunoassay. Statistical analysis of the results was performed using the Statistica 8.0 software. Results. The levels of IgG-Bp >7 and IgG-Es>6 were higher in patients with colorectal cancer than in healthy controls (66 % vs 25 %, p<0.0001, OR=5.9 and 58 % vs s 45 %, p=0.002, OR=1.7, respectively). The individual antibody ratios of IgG-Bp/IgG-Es >1, IgG-Bp/IgG-Pg>1.5, and IgG-Es/IgG-Pg>1.5 were also higher in patients with colorectal cancer than in healthy controls (74 % vs 34 %, p<0.0001, OR=5.6; 75 % vs 28 %, p<0.0001, and 58 % vs 38 %, p<0.0001, OR=2.3, respectively). Compared to healthy controls, breast cancer patients had higher values of IgG-Bp >6 (57 % vs 33 %, p<0.0001, OR=2.7) and IgG-Es>5 (62 % vs 53 %, p=0.003, or=1.4) and ratios of IgG-Bp/IgG-Es>1 (55 % vs 34 %, p<0.0001, or=2.4), IgG-Bp/IgG-Pg>1.3 (71 % vs 36 %, p<0.0001, or=4.5) and IgG-Es/IgG-Pg>1.4 (62 % vs 44 %, p<0.0001, or=2.1). Compared to breast cancer patients, colorectal cancer patients had higher values of IgG-Bp>7 (66 % vs 50 %, p<0.0001) and the ratios of igg-Bp/IgG-Es >1 (74 % vs 55 %, p<0.0001) and IgG-Bp/IgG-Pg>1.5 (76 % vs 60 %, p<0.0001). Conclusion. IgG-Bp, IgG-Es, and IgG-Pg immunoassay could serve as a screening tool to identify population at risk of colorectal and breast cancers.
Publisher
Tomsk Cancer Research Institute
Reference16 articles.
1. Malignant tumors in Russia in 2019 (morbidity and mortality). Ed. by A.D. Kaprin, V.V. Starinsky, A.O. Shakhzadova. Moscow, 2020. 214 p. (in Russian).
2. Khudolei V.V. Carcinogens: characteristics, patterns, mechanisms of action. St. Petersburg, 1990, 419 p. (in Russian).
3. Gunter M.J., Divi R.L., Kulldorff M., Vermeulen R., Haverkos K.J., Kuo M.M., Strickland P., Poirier M.C., Rothman N., Sinha R. Leukocyte polycyclic aromatic hydrocarbon-DNA adduct formation and colorectal adenoma. Carcinogenesis. 2007; 28(7): 1426–9. doi: 10.1093/carcin/bgm022.
4. Jamin E.L., Riu A., Douki T., Debrauwer L., Cravedi J.P., Zalko D., Audebert M. Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis. PLoS One. 2013; 8(3). doi: 10.1371/journal.pone.0058591.
5. Harris D.L., Washington M.K., Hood D.B., Roberts L.J., Ramesh A. Dietary fat-influenced development of colon neoplasia in Apc Min mice exposed to benzo(a)pyrene. Toxicol Pathol. 2009; 37(7): 938–46. doi: 10.1177/0192623309351722.