1. heavy metal transporter PDRlike ABC transporter-related Glyma.03G168000; glucose hexaphosphate-related Glyma.06G032451; and phosphatase-related Glyma.20G000800. When treated with B. subtilis-GmRALF22;Glyma.07G051500, Glyma.15G179600, Glyma.07G041400 and Glyma.08G011300 were up
2. GmRALF genes have tissuespecific expression patterns, indicating their crucial role in root, nodule, flower, and seed development (Fig. 3A). Hence, functional studies on the RALF genes that are highly expressed in soybean nodules can shed fresh light on their role in regulating nodulation and nitrogen nutrition;Collier;AtRALF31 have been reported. Moreover, three conserved motifs are found in the soybean RALFs, and GmRALF22 contains all three motifs (Fig.S1),2012
3. GmRALF6, and GmRALF25 were significantly upregulated by LP in leaves;B;GmRALF,2022
4. In line with this, we discovered that the promoter region (-2000 bp) of GmRALF5, GmRALF6, GmRALF10, GmRALF11, GmRALF20, GMRALF22, and GmRALF25 contain the P1BS ciselement (Fig.4A). GmPHR1 has been found to be P signaling-related transcription factor. Dual-luciferase assay and EMSA analysis demonstrated that GmPHR1 directly binds to the P1BS element of GmRALF22 promoter and stimulates its expression (Fig. 5);Yang;AtRALF33 and AtRALF34 containing P1BS elements are responsive to LP conditions,2015
5. Family-wide evaluation of rapid alkalinization factor peptides[J];A Abarca;Plant Physiology,2021