A technique for rearing germfree piglets obtained without surgery

Author:

Ratcliffe B.1,Fordham J. P.1

Affiliation:

1. Agricultural and Food Research Council Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, United Kingdom

Abstract

A relatively simple procedure is described for obtaining germfree piglets which does not involve hysterectomy or hysterotomy. Newborn pigs were delivered into an isolator and their freedom from microbial contamination was ensured by applying bactericidal solutions externally and a combination of antibiotics in solution per os. 40 piglets so derived have been maintained free from detectable micro-organisms, some for up to 140 days. Equipment is described which allowed the long-term holding of these animals so that nutritional balance studies could be completed.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Production and rearing of germ-free X-SCID pigs;Experimental Animals;2018

2. Intestinal MicrobiOMICS to Define Health and Disease in Human and Mice;Current Pharmaceutical Biotechnology;2012-04-04

3. Energy values of lactitol and lactulose as determined with miniature pigs and growing rats;Journal of the Science of Food and Agriculture;1990

4. Effect of soya-bean protein on the ability of gnotobiotic pig intestine to digest and absorb nutrients;The Journal of Agricultural Science;1989-02

5. Gnotobiotic Animals in Nutrition Research;New Developments in Biosciences: Their Implications for Laboratory Animal Science;1988

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