Effect of soya-bean protein on the ability of gnotobiotic pig intestine to digest and absorb nutrients

Abstract

SummaryGnotobiotic pigs were fed control and soya-bean protein containing diets in an attempt to identify direct effects of this protein on intestinal structure and function.Feeding diets containing soya-bean protein for 4 days to 21-day-old gnotobiotic pigs increased crypt depth in the lower half of the small intestine. This increase was accompanied by a corresponding reduction in lactase activity, brought about mainly by a decrease in the apparent rate at which this enzyme appeared in the luminal membrane of developing enterocytes. Sucrase and maltase II and III activities increased slightly in tissue taken from pigs fed soya-bean protein. Alanine transport measured in the presence and absence of Na remained unaffected by change of diet.The structure and function of control gnotobiotic pig intestine differed from that found in normal unweaned piglets. The possible source of these differences and the probable role played by enteric microflora in amplifying initial effects of soya-bean protein on intestinal function is discussed.