Affiliation:
1. Institute of Experimental Medicine; Military Medical Academy named after S.M. Kirov of the Ministry of Defense of Russia
2. Smolensk State Medical University of the Ministry of Health Care of Russia
Abstract
The effect of nifedipine, a calcium channel blocker (CCB), at a dose of 7.5 mg/kg on the energy metabolism of rabbit cardiomyocytes was evaluated in a vibration-mediated model of cellular hypoxia (56 sessions of vibration 44 Hz, amplitude 0.5 mm). The energy metabolism of native heart mitochondria in a 30% tissue homogenate was assessed using a polarographic method, by recording the rate of oxygen uptake by mitochondria at 37°C in 1 ml of a saline incubation medium, equilibrated with atmospheric oxygen. In the animals exposed to vibration against the background of CCB, the rate of endogenous respiration (Ve) remained at the level of intact animals, with the amytal sensitivity increasing by 39% (p<0.05) and the malonate sensitivity decreasing by 40% (p<0.05). The malate oxidase activity at rest increased, and hyperactivation of the endogenous succinic acid oxidation system decreased as compared to the indices of the animals exposed to vibration without pharmacological protection. The observed results indicate the cardioprotective effect of nifedipine, which prevented the development of cardiomyocyte necrosis.
Publisher
Scientific Center for Biomedical Technologies of the Federal Medical-Biological Agency
Reference25 articles.
1. Belosludtsev K.N., Dubinin M.V., Belosludtseva N.V., Mironova G.D. Transport ionov Са2+ mitokhondriyami: mekhanizmy, molekulyarnye struktury i znachenie dlya kletki [Mitochondrial Са2+ transport: Mechanisms, molecular structures, and role in cells]. Biokhimiya [Biochemistry]. 2019;84(6):759–775. (In Russian). DOI: 10.1134/S0320972519060022.
2. Volchegorskiy I.A., Dolgushin I.I., Kolesnikov O.A., Tseylikman V.E. Eksperimental’noe modelirovanie i laboratornaya otsenka adaptivnykh reaktsiy organizma [Experimental modeling and laboratory assessment of the adaptive reactions of the body]. Chelyabinsk:Chelyabinsk State Pedagogical University Publ., 2000:167. (In Russian).
3. Vorob’eva V.V., Shabanov P.D. Vibratsionnaya model’ gipoksicheskogo tipa kletochnogo metabolizma, otsenennaya na kardio miotsitakh krolika [Vibration model for hypoxic type of cell metabolism evaluated on rabbit cardiomyocytes]. Bulletin of Experimental Biology and Medicine. 2009;147(6):712–715. (In Russian).
4. Karkishchenko N.N. Osnovy biomodelirovaniya [The basics of biomodelling]. Moscow: VPK Publ., 2005:608. (In Russian).
5. Kiryakov V.A., Pavlovskaya N.A., Lapko I.V., Bogatyreva I.A., Antoshina L.I., Oshkoderov O.A. Vozdeystvie proizvodstvennoy vibratsii na organizm cheloveka na molekulyarnokletochnom urovne [Impact of occupational vibration on molecular and cell level of human body]. Meditsina truda i promyshlennaya ekologiya [Russian Journal of Occupational Health and Industrial Ecology]. 2018;9:34–43. (In Russian). DOI: 10.31089/1026-9428-2018-9-34-43.
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