Paralytic hypo-energetic state facilitates anoxia tolerance despite ionic imbalance in adult Drosophila melanogaster

Abstract

Oxygen limitation plays a key role in many pathologies; yet, we still lack a fundamental understanding of the mechanisms responsible for variation in anoxia tolerance. Most vertebrate studies suggest that anoxia tolerance involves the ability to maintain cellular ATP despite the loss of aerobic metabolism. However, insects such as adult D. melanogaster are able to survive long periods of anoxia (LT50: ∼8 h) in a hypo-energetic state characterized by low [ATP]. In this study, we tested for possible mechanisms that allow D. melanogaster adults to survive long periods of anoxia. Adults are paralyzed within 30 sec, and after two hours of anoxia ATP was 3% of normal, [K+]o increased threefold, pH dropped 1 unit, yet survival was 100%. With 0.5-6 h of anoxia, adults maintained low but constant ATP levels while [K+]o and pHo continued to change. When returned to normoxia, adults restore [K+]o and activity. With longer durations of anoxia, ATP levels decreased and [K+]o rose further, and both correlated tightly with decreased survival. This response contrasts with the anoxia-sensitive larval stage (LT50: ∼1 h). During anoxia, larvae attempt escape for up to 30 min and after two hours of anoxia, ATP was <1% of resting, [K+]o increased by 50%, hemolymph pH fell by 1 unit, and survival was zero. The superior anoxia tolerance of adult D. melanogaster appears to be due to the capacity to maintain a paralytic hypometabolic state with low but non-zero ATP levels, and to be able to tolerate extreme extracellular ionic variability.