Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts
Author:
Mingle Lisa A.1, Okuhama Nataly N.1, Shi Jian1, Singer Robert H.2, Condeelis John2, Liu Gang1
Affiliation:
1. Center for Cell Biology and Cancer Research, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208, USA 2. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Abstract
The actin-related protein 2/3 (Arp2/3) complex is a crucial actin polymerization nucleator and is localized to the leading protrusions of migrating cells. However, how the multiprotein complex is targeted to the protrusions remains unknown. Here, we demonstrate that mRNAs for the seven subunits of the Arp2/3 complex are localized to the protrusions in fibroblasts, supporting a hypothesis that the Arp2/3 complex is targeted to its site of function by mRNA localization. Depletion of serum from culture medium inhibits Arp2/3-complex mRNA localization to the protrusion, whereas serum stimulation leads to significant mRNA localization within 30 minutes. The effect of serum suggests that Arp2/3-complex mRNA localization is a cellular response to extracellular stimuli. The localization of the Arp2/3 complex mRNAs is dependent on both actin filaments and microtubules, because disruption of either cytoskeletal system (with cytochalasin D and colchicine, respectively) inhibited the localization of all seven subunit mRNAs. In addition, myosin inhibitors significantly inhibit Arp2 mRNA localization in chicken embryo fibroblasts, suggesting a myosin motor dependent mechanism for Arp2/3-complex mRNA localization.
Publisher
The Company of Biologists
Reference43 articles.
1. Bailly, M., Macaluso, F., Cammer, M., Chan, A., Segall, J. E. and
Condeelis, J. S. (1999). Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation. J. Cell Biol.145, 331-345. 2. Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L. M., Segall,
J. E. and Condeelis, J. (2001). The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Curr. Biol.11, 620-625. 3. Barbarese, E., Koppel, D. E., Deutscher, M. P., Smith, C. L., Ainger, K.,
Morgan, F. and Carson, J. H. (1995). Protein translation components are colocalized in granules in oligodendrocytes. J. Cell Sci.108, 2781-2790. 4. Bassell, G. J., Oleynikov, Y. and Singer, R. H. (1999). The travels of mRNAs through all cells large and small. FASEB J.13, 447-454. 5. Berleth, T., Burri, M., Thoma, G., Bopp, D., Richstein, S., Frigerio, G.,
Noll, M. and Nusslein-Volhard, C. (1988). The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo. EMBO J.7, 1749-1756.
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