Extracellular glucose can fuel metabolism in RBCs from high glycemic Atlantic cod (Gadus morhua) but not low glycemic short-horned sculpin (Myoxocephalus scorpius)

Abstract

Abstract Energy metabolism was assessed in RBCs from Atlantic cod and short-horned sculpin, two species that have markedly different levels of blood glucose. The objective was to determine if the level of extracellular glucose impacts rates of glucose metabolism. Blood glucose level was 2.5 mmol l-1 in Atlantic cod and 0.2 mmol l-1 in short-horned sculpin, respectively. Oxygen consumption, lactate production, and glucose utilization were measured in whole blood and related to g RBC. Glucose utilization was assessed by measuring both glucose disappearance and by the production of 3H2O from [2-3H]-glucose. RBCs from both species have an aerobic based metabolism. In Atlantic cod, extracellular glucose is sufficient to provide the sum of glucosyl equivalents to support both oxidative metabolism and lactate production. In contrast, extracellular glucose can account for only 10% of the metabolic rate in short-horned sculpin RBCs. In both species, about 70% of glucose enters the RBCs via facilitated transport. The difference in rates of extracellular glucose utilization is related to the extremely low levels of blood glucose in short-horned sculpin. In this species energy metabolism by RBCs must be supported by alternative fuels.