Early development and molecular plasticity in the Mediterranean sea urchinParacentrotus lividusexposed to CO2-driven acidification

Author:

Martin Sophie123,Richier Sophie45,Pedrotti Maria-Luiza45,Dupont Sam6,Castejon Charlotte1,Gerakis Yannis45,Kerros Marie-Emmanuelle45,Oberhänsli François1,Teyssié Jean-Louis1,Jeffree Ross1,Gattuso Jean-Pierre45

Affiliation:

1. Marine Environment Laboratories, International Atomic Energy Agency, 4 Quai Antoine Ier, 98000 Principality of Monaco

2. CNRS, Laboratoire Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff Cedex, France

3. Université Pierre et Marie Curie, Paris 06, Laboratoire Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff Cedex, France

4. CNRS-INSU, Laboratoire d'Océanographie de Villefranche-sur-Mer, BP 28, 06234 Villefranche-sur-Mer Cedex, France

5. Université Pierre et Marie Curie, Paris 06, Observatoire Océanologique de Villefranche, 06230 Villefranche-sur-Mer Cedex, France

6. Department of Marine Ecology, University of Göteborg, The Sven Lovén Centre for Marine Science, Kristineberg, 45034 Fiskebäckskil, Sweden

Abstract

SUMMARYOcean acidification is predicted to have significant effects on benthic calcifying invertebrates, in particular on their early developmental stages. Echinoderm larvae could be particularly vulnerable to decreased pH, with major consequences for adult populations. The objective of this study was to understand how ocean acidification would affect the initial life stages of the sea urchin Paracentrotus lividus, a common species that is widely distributed in the Mediterranean Sea and the NE Atlantic. The effects of decreased pH (elevated PCO2) were investigated through physiological and molecular analyses on both embryonic and larval stages. Eggs and larvae were reared in Mediterranean seawater at six pH levels, i.e. pHT 8.1, 7.9, 7.7, 7.5, 7.25 and 7.0. Fertilization success, survival, growth and calcification rates were monitored over a 3 day period. The expression of genes coding for key proteins involved in development and biomineralization was also monitored. Paracentrotus lividus appears to be extremely resistant to low pH, with no effect on fertilization success or larval survival. Larval growth was slowed when exposed to low pH but with no direct impact on relative larval morphology or calcification down to pHT 7.25. Consequently, at a given time, larvae exposed to low pH were present at a normal but delayed larval stage. More surprisingly, candidate genes involved in development and biomineralization were upregulated by factors of up to 26 at low pH. Our results revealed plasticity at the gene expression level that allows a normal, but delayed, development under low pH conditions.

Publisher

The Company of Biologists

Subject

Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3