Affiliation:
1. Howard Hughes Medical Institute, Department of Molecular Biology,Princeton University, Princeton, NJ 08544, USA
Abstract
Heterogeneous nuclear ribonucleoproteins, hnRNPs, are RNA-binding proteins that play crucial roles in controlling gene expression. In Drosophilaoogenesis, the hnRNP Squid (Sqd) functions in the localization and translational regulation of gurken (grk) mRNA. We show that Sqd interacts with Hrb27C, an hnRNP previously implicated in splicing. Like sqd, hrb27C mutants lay eggs with dorsoventral defects and Hrb27C can directly bind to grk RNA. Our data demonstrate a novel role for Hrb27C in promoting grk localization. We also observe a direct physical interaction between Hrb27C and Ovarian tumor (Otu), a cytoplasmic protein implicated in RNA localization. We find that some otu alleles produce dorsalized eggs and it appears that Otu cooperates with Hrb27C and Sqd in the oocyte to mediate proper grklocalization. All three mutants share another phenotype, persistent polytene nurse cell chromosomes. Our analyses support dual cooperative roles for Sqd,Hrb27C and Otu during Drosophila oogenesis.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Reference46 articles.
1. Carson, J. H., Cui, H., Krueger, W., Schlepchenko, B., Brumwell,C. and Barbarese, E. (2001). RNA trafficking in oligodendrocytes. Results Probl. Cell. Differ.34, 69-81.
2. Chou, T. B. and Perrimon, N. (1992). Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila. Genetics131,643-653.
3. Chou, T. B. and Perrimon, N. (1996). The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster. Genetics144,1673-1679.
4. Dej, K. J. and Spradling, A. C. (1999). The endocycle controls nurse cell polytene chromosome structure during Drosophila oogenesis. Development126,293-303.
5. Dreyfuss, G., Matunis, M. J., Pinol-Roma, S. and Burd, C. G.(1993). hnRNP proteins and the biogenesis of mRNA. Annu. Rev. Biochem.62,289-321.
Cited by
111 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献