A high-throughput screen identifies RNA binding proteins that affect fertility inCaenorhabditis elegansand reveals a functional relationship between ADR-2 and SQD-1

Author:

Erdmann Emily A.ORCID,Forbes Melanie,Becker Margaret,Perez Sarina,Skelly Mary,Hundley Heather A.ORCID

Abstract

ABSTRACTRNA binding proteins play essential roles in coordinating germline gene expression and development in all organisms. Characterization of gross fertility defects, such as sterility, has identified RNA binding proteins that are critical regulators of germline gene expression; however, broader screens for RNA binding proteins involved in specific reproductive processes are lacking. Here, a reverse genetic screen was performed to identify RNA binding proteins that impact yolk and embryo production inCaenorhabditis eleganshermaphrodites. This screen makes use of animals expressing a vitellogenin (yolk protein) fusion with green fluorescent protein, in a genetic background that corrects for a previously identified fertility defect in this strain. From this screen, we identified 23 RNA binding proteins that regulate embryo production inCaenorhabditis elegans. This screen lays groundwork for future interrogations into the molecular roles of these proteins in yolk production and embryogenesis. Additionally, the screen uncovered a genetic interaction between ADR-2, a member of the Adenosine DeAminase Acting on RNA (ADAR) family, and SQD-1, a member of the heterogenous nuclear ribonucleoprotein (hnRNP) family. Transcriptome-wide assessment in animals depleted ofsqd-1revealed over 8000 misregulated transcripts, suggesting SQD-1 is a major regulator of gene expression. Consistent with this, microscopy and reproductive assays reveal thatsqd-1is essential for oogenesis. In the absence ofadr-2, the effects of loss ofsqd-1on gene expression are attenuated, as well as the defects in oogenesis. Together, these data indicate that both ADR-2 and SQD-1 are important regulators of germline gene expression and oogenesis.Article SummaryThe molecular information that controls early development is RNA. Interactions between RNA and RNA binding proteins are critical for successful reproduction across species. In this study, over 250 RNA binding proteins were assessed for effects on yolk and embryo production inCaenorhabditis elegans. Twenty-three regulators of these important reproductive steps were identified, including 7 novel regulators. This screen also revealed a genetic interaction between two RNA binding proteins, SQD-1 and ADR-2, which impacts germline gene expression, oogenesis, and fertility.

Publisher

Cold Spring Harbor Laboratory

Reference94 articles.

1. RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let- 7 miRNA pathways in Caenorhabditis elegans

2. The role of RNA-binding proteins in orchestrating germline development in Caenorhabditis elegans;Front Cell Dev Biol,2022

3. Angeles-Albores D , Lee R , Chan J , Sternberg P. 2018. Two new functions in the WormBase Enrichment Suite. MicroPubl Biol 2018.

4. Tissue enrichment analysis for C. elegans genomics

5. mRNA Editing, Processing and Quality Control in Caenorhabditis elegans

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3