Trp53 ablation fails to prevent microcephaly in mouse pallium with impaired minor intron splicing

Author:

White Alisa K.1,Baumgartner Marybeth2,Lee Madisen F.1,Drake Kyle D.1ORCID,Aquino Gabriela S.1,Kanadia Rahul N.13ORCID

Affiliation:

1. Physiology and Neurobiology Department, University of Connecticut, Storrs, CT 06269, USA

2. Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA

3. Institute of Systems Genomics, University of Connecticut, Storrs, CT 06269, USA

Abstract

ABSTRACT Minor spliceosome inhibition due to mutations in RNU4ATAC are linked to primary microcephaly. Ablation of Rnu11, which encodes a minor spliceosome snRNA, inhibits the minor spliceosome in the developing mouse pallium, causing microcephaly. There, cell cycle defects and p53-mediated apoptosis in response to DNA damage resulted in loss of radial glial cells (RGCs), underpinning microcephaly. Here, we ablated Trp53 to block cell death in Rnu11 cKO mice. We report that Trp53 ablation failed to prevent microcephaly in these double knockout (dKO) mice. We show that the transcriptome of the dKO pallium was more similar to the control compared with the Rnu11 cKO. We find aberrant minor intron splicing in minor intron-containing genes involved in cell cycle regulation, resulting in more severely impaired mitotic progression and cell cycle lengthening of RGCs in the dKO that was detected earlier than in the Rnu11 cKO. Furthermore, we discover a potential role of p53 in causing DNA damage in the developing pallium, as detection of γH2aX+ was delayed in the dKO. Thus, we postulate that microcephaly in minor spliceosome-related diseases is primarily caused by cell cycle defects.

Funder

National Institutes of Health

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

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