FAM83G/PAWS1 controls cytoskeletal dynamics and cell migration through association with the SH3 adaptor CD2AP

Author:

Cummins Timothy D.12,Wu Kevin Z. L.1,Bozatzi Polyxeni1,Dingwell Kevin S.3,Macartney Thomas J.1,Wood Nicola T.1,Varghese Joby1,Gourlay Robert1,Campbell David G.1,Prescott Alan4,Griffis Eric56ORCID,Smith James C.3,Sapkota Gopal P.1ORCID

Affiliation:

1. Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, Dundee, Scotland, UK

2. Current Address: Division of Nephrology and Hypertension, Clinical Proteomics Center, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA

3. The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK

4. Cell Signalling and Immunology, University of Dundee, Dundee DD1 5EH, Scotland, UK

5. Centre for Gene Regulation and Expression, University of Dundee, Dundee, Scotland, UK

6. Current Address: NINDS/NIH, Bethesda, MD, 20892, USA

Abstract

Our previous studies of PAWS1 (Protein Associated With SMAD1) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1 knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the cytoskeletal machinery, including actin and focal adhesion dynamics, and cell migration. Confocal microscopy and live cell imaging of actin in U2OS cells indicate that PAWS1 is also involved in cytoskeletal dynamics and organization. Loss of PAWS1 causes severe defects in F-actin organization and distribution as well as in lamellipodial organization, resulting in impaired cell migration. PAWS1 interacts in a dynamic fashion with the actin/cytoskeletal regulator CD2AP at lamellae, suggesting that its association with CD2AP controls actin organization and cellular migration. Genetic ablation of CD2AP from U2OS cells instigates actin and cell migration defects reminiscent of those seen in PAWS1 knockout cells.

Funder

Medical Research Council

Cancer Research UK

Wellcome Trust

Publisher

The Company of Biologists

Subject

Cell Biology

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