A High-Throughput Microdroplet-Based Single Cell Transfection Method for Gene Knockout Based on the CRISPR/Cas9 System
Author:
Affiliation:
1. Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, SAR, China
2. Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, SAR, China
Funder
Research Grant Council of Hong Kong, SAR, China
Key Program of National Natural Science Foundation of China
City University of Hong Kong
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Link
http://xplorestaging.ieee.org/ielx7/7728/10483562/10459222.pdf?arnumber=10459222
Reference53 articles.
1. Cellular Analysis Using Microfluidics
2. Live-cell imaging to compare the transfection and gene silencing efficiency of calcium phosphate nanoparticles and a liposomal transfection agent
3. Conceptual and technical aspects of transfection and gene delivery
4. Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery
5. A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
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