α-Endosulfine is a conserved protein required for oocyte meiotic maturation in Drosophila
Author:
Von Stetina Jessica R.1, Tranguch Susanne1, Dey Sudhansu K.123, Lee Laura A.1, Cha Byeong1, Drummond-Barbosa Daniela1
Affiliation:
1. Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. 2. Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA. 3. Department of Pharmacology, Vanderbilt University Medical Center, Nashville,TN 37232, USA.
Abstract
Meiosis is coupled to gamete development and must be well regulated to prevent aneuploidy. During meiotic maturation, Drosophila oocytes progress from prophase I to metaphase I. The molecular factors controlling meiotic maturation timing, however, are poorly understood. We show that Drosophila α-endosulfine (endos) plays a key role in this process. endos mutant oocytes have a prolonged prophase I and fail to progress to metaphase I. This phenotype is similar to that of mutants of cdc2 (synonymous with cdk1) and of twine, the meiotic homolog of cdc25, which is required for Cdk1 activation. We found that Twine and Polo kinase levels are reduced in endos mutants, and identified Early girl (Elgi), a predicted E3 ubiquitin ligase, as a strong Endos-binding protein. In elgi mutant oocytes, the transition into metaphase I occurs prematurely, but Polo and Twine levels are unaffected. These results suggest that Endos controls meiotic maturation by regulating Twine and Polo levels, and, independently, by antagonizing Elgi. Finally, germline-specific expression of the humanα-endosulfine ENSA rescues the endos mutant meiotic defects and infertility, and α-endosulfine is expressed in mouse oocytes, suggesting potential conservation of its meiotic function.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Reference57 articles.
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