From Cells to Cell-Free Platforms

Abstract

Cell-free systems are powerful tools in biotechnology, allowing researchers to study and manipulate biological processes outside of intact cells. This chapter provides a comprehensive introduction to cell-free systems, covering their principles, historical background, preparation methods, and various approaches used in research and applications. It emphasizes the advantages of cell-free systems, their historical development, and the different methods for preparing cell extracts or purified components. The chapter explores approaches like extracts-based cell-free systems and the PURE system, discussing their methodologies and applications. It also highlights the key components and factors influencing the efficiency of cell-free systems. Therefore, this chapter serves as a foundation for understanding the potential of cell-free systems in biotechnology research.