A technique to increase protein yield in a rabbit reticulocyte lysate translation system-Reference-Cited by-同舟云学术

A technique to increase protein yield in a rabbit reticulocyte lysate translation system

Published:2014-01 Issue:1 Volume:56 Page:36-39
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Anastasina Maria¹,Terenin Ilya²,Butcher Sarah J³,Kainov Denis E¹

Affiliation:

1. Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Finland

2. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russian Federation

3. Institute of Biotechnology, University of Helsinki, Finland

Abstract

Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show that simultaneous addition of influenza A virus NS1 protein and inclusion of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) in the target mRNA facilitate translation initiation and increase protein yield over 10-fold, improving the translation capacity of RRL.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/000114125

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