Affiliation:
1. California Institute of Technology, Pasadena, CA, USA
Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.
Subject
General Biochemistry, Genetics and Molecular Biology,Biotechnology
Cited by
30 articles.
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